show Abstracthide AbstractSome Escherichia coli strains have been shown to provide beneficial effects on human and animal health. Six of those strains are contained in the probiotic drug Symbioflor2, provided by SymbioPharm (Herborn-Hoerbach, Germany). While this product is used in humans for decades without severe side effects and in this way considered as safe, it is an essential step to ensure complete safety by evaluating their whole genomic content.